Protein expression

      An antigen is a molecule recognized by the immune system. At the molecular level, an antigen is characterized by its ability to be "bound" at the antigen-binding site of an antibody. Immunogenicity is the ability to induce a humoral and/or cell-mediated immune response. Antigenicity is the ability to combine specifically with the final products of the immune response(i.e. secreted antibodies and/or surface receptors on T-cells). Although all molecules that have the property of immunogenicity also have the property of antigenicity, the reverse is not true. Antigens are usually proteins that are too large to bind as a whole to any receptor, only specific segments that form the antigen bind with a specific receptor. Such segments are called epitopes.A linear or a sequential epitope is an epitope that is recognized by antibodies by its linear sequence of amino acids, or primary structure. In contrast, antibodies recognize a conformational epitope that has a specific three-dimensional shape and its protein structure. Design and select a suitable antigen is a key to produce antibodies for various applications.
      Trigoats has the antigen leading technology and advanced equipment, can provide customers with: (1) Protein expression and purification in prokaryotic system; (2) Peptide synthesis and conjugation.


Service Code
Brief Description
gene synthesis
Oligo DNA sequence synthesis
2-4 weeks
subclone(expression plasmid construct)
PCR,enzyme restrict, ligate,transform,sequencing
2-3 weeks
Prokaryotic expression and purification
induction,optimation, expression and purification
3-4 weeks
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